Examination of the metabolic status of rat air pouch inflammatory exudate by high field proton NMR spectroscopy

Citation
A. Claxson et al., Examination of the metabolic status of rat air pouch inflammatory exudate by high field proton NMR spectroscopy, BBA-MOL BAS, 1454(1), 1999, pp. 57-70
Citations number
20
Categorie Soggetti
Medical Research General Topics
Journal title
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
ISSN journal
09254439 → ACNP
Volume
1454
Issue
1
Year of publication
1999
Pages
57 - 70
Database
ISI
SICI code
0925-4439(19990531)1454:1<57:EOTMSO>2.0.ZU;2-5
Abstract
High field proton (H-1) NMR spectroscopy was employed to investigate the me tabolic status of rat air pouch inflammatory exudates obtained subsequent t o the induction of inflammation with carrageenan, and the H-1 NMR profiles of these fluids were compared and contrasted with those of inflammatory hum an synovial fluid, rat plasma and human serum. The characteristic biochemic al features obtained from H-1 NMR analysis of these exudates consisted of ( 1) substantially elevated levels of lactate (11.40 +/- 1.46 x 10(-3) mol dm (-3) for samples collected at a time point of 24 h post induction) with lit tle or no NMR-detectable glucose, data consistent with a hypoxic environmen t and consequent anaerobic metabolism in the inflamed air pouch, and (2) hi gh levels of the ketone body 3-D-hydroxybutyrate, providing evidence for an increased utilization of fats for energy by lymphocytes, the predominant l eucocytes present in this environment. These phenomena represent a patholog ical extreme of the abnormal metabolic status of inflammatory human synovia l fluids. (C) 1999 Elsevier Science B.V. All rights reserved.