A. Claxson et al., Examination of the metabolic status of rat air pouch inflammatory exudate by high field proton NMR spectroscopy, BBA-MOL BAS, 1454(1), 1999, pp. 57-70
Citations number
20
Categorie Soggetti
Medical Research General Topics
Journal title
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
High field proton (H-1) NMR spectroscopy was employed to investigate the me
tabolic status of rat air pouch inflammatory exudates obtained subsequent t
o the induction of inflammation with carrageenan, and the H-1 NMR profiles
of these fluids were compared and contrasted with those of inflammatory hum
an synovial fluid, rat plasma and human serum. The characteristic biochemic
al features obtained from H-1 NMR analysis of these exudates consisted of (
1) substantially elevated levels of lactate (11.40 +/- 1.46 x 10(-3) mol dm
(-3) for samples collected at a time point of 24 h post induction) with lit
tle or no NMR-detectable glucose, data consistent with a hypoxic environmen
t and consequent anaerobic metabolism in the inflamed air pouch, and (2) hi
gh levels of the ketone body 3-D-hydroxybutyrate, providing evidence for an
increased utilization of fats for energy by lymphocytes, the predominant l
eucocytes present in this environment. These phenomena represent a patholog
ical extreme of the abnormal metabolic status of inflammatory human synovia
l fluids. (C) 1999 Elsevier Science B.V. All rights reserved.