Effects of thymidine and 2-D-deoxyglucose on insulin-like growth factor binding protein-1 (IGFBP-1) and erythropoietin secretion in HepG2 cells

Effects of thymidine and 2-D-deoxyglucose on insulin-like growth factor-bin ding protein-1 (IGFBP-1) and erythropoietin (Ep) secretion were investigate d in HepG2 cells. The maximal insulin-like growth factor-I (IGF-I) binding capacity was obtained at confluency, while Ep production was dominant in th e proliferating stage and declined at confluency. Treatment with high conce ntraion of thymidine inhibited Ep production as well as proliferation of He pG2 cells, while IGFBP-1 production was increased by thymidine treatment. A ddition of 2-D-deoxyglucose decreased viable cell number and Ep production and increased medium IGFBP-1 levels. These findings suggest that IGFBP-1 an d Ep productions are linked to cell proliferation and that IGFBP-1 producti on is dominant in quiescent cells in HepG2 cells.