J. Tanaka et al., Effects of thymidine and 2-D-deoxyglucose on insulin-like growth factor binding protein-1 (IGFBP-1) and erythropoietin secretion in HepG2 cells, BIOMED RES, 19(4), 1998, pp. 289-292
Effects of thymidine and 2-D-deoxyglucose on insulin-like growth factor-bin
ding protein-1 (IGFBP-1) and erythropoietin (Ep) secretion were investigate
d in HepG2 cells. The maximal insulin-like growth factor-I (IGF-I) binding
capacity was obtained at confluency, while Ep production was dominant in th
e proliferating stage and declined at confluency. Treatment with high conce
ntraion of thymidine inhibited Ep production as well as proliferation of He
pG2 cells, while IGFBP-1 production was increased by thymidine treatment. A
ddition of 2-D-deoxyglucose decreased viable cell number and Ep production
and increased medium IGFBP-1 levels. These findings suggest that IGFBP-1 an
d Ep productions are linked to cell proliferation and that IGFBP-1 producti
on is dominant in quiescent cells in HepG2 cells.