The preparation, crystallization, and preliminary X-ray study of a Fab-fragment of monoclonal antibody to human interleukin-2 and its complex with anantigenic peptide

Authors
Mikhailova, IY Mareeva, TY Tsygannik, IN Mikhaleva, II Onoprienko, LV Vikhrov, AA Markvicheva, EA Pangborn, W Duax, W Nesmeyanov, VA Pletnev, VZ
Citation
Iy. Mikhailova et al., The preparation, crystallization, and preliminary X-ray study of a Fab-fragment of monoclonal antibody to human interleukin-2 and its complex with anantigenic peptide, BIOORG KHIM, 25(4), 1999, pp. 247-252
Citations number
17
Categorie Soggetti
Chemistry & Analysis
Journal title
BIOORGANICHESKAYA KHIMIYA
ISSN journal
01323423 → ACNP
Volume
25
Issue
4
Year of publication
1999
Pages
247 - 252
Database
ISI
SICI code
0132-3423(199904)25:4<247:TPCAPX>2.0.ZU;2-J
Abstract
Antigen-binding fragments (Fab) of mouse monoclonal antibodies to human int erleukin-2 were obtained in preparative quantities by a modified procedure. These Fab-fragments were shown to be homogeneous according to the isoelect ric focusing method. Various monocrystals of these free Fab-fragments and t heir complexes with the antigenic peptide corresponding to the 59-72 sequen ce of interleukin-2 were obtained. These were shown to be suitable for X-ra y and were preliminarily studied by X-ray.