Sorption on synthetic membranes and immunogenic properties of aprotinin intermolecularly crosslinked by glutaraldehyde

A procedure for the intermolecular crosslinking of aprotinin (natural polyp eptide, an inhibitor of serine proteases) by glutaraldehyde was proposed. T his autoconjugation of aprotinin increased its immunogenicity and the effic iency of sorption on nitrocellulose and polyvinylidene difluoride membranes . The immunization of animals by the autoconjugated aprotinin induced the p roduction of antibodies that reacted with both conjugated and monomeric apr otinin. The properties of the crosslinked aprotinin are promising for the e nhancement of sensitivity through its use in EIA and immunoblotting membran e methods that employ aprotinin and anti-aprotinin antibodies.