Heterologous expression of murine lymphotoxins in Escherichia coli and thepreparation of antibodies

Authors
Korobko, VG Boichenko, VE Kuprash, DV Turetskaya, RL Nedospasov, SA
Citation
Vg. Korobko et al., Heterologous expression of murine lymphotoxins in Escherichia coli and thepreparation of antibodies, BIOORG KHIM, 25(4), 1999, pp. 270-274
Citations number
23
Categorie Soggetti
Chemistry & Analysis
Journal title
BIOORGANICHESKAYA KHIMIYA
ISSN journal
01323423 → ACNP
Volume
25
Issue
4
Year of publication
1999
Pages
270 - 274
Database
ISI
SICI code
0132-3423(199904)25:4<270:HEOMLI>2.0.ZU;2-Q
Abstract
Genes encoding fragments of polypeptide chains of murine lymphotoxins (LT), namely, LT-alpha truncated from the N-terminus and the LT-beta extracellul ar domain, containing N-terminal hepta- and hexahistidine epitopes, respect ively, were expressed in E. coli cells. The recombinant proteins purified b y metallochelate chromatography were used to obtain polyclonal antibodies t hat specifically recognize murine LT.