A liquid phase blocking ELISA for the detection of antibodies against infectious bronchitis virus

A liquid phase blocking ELISA (LPB-ELISA) was developed for the detection a nd measurement of antibodies against infectious bronchitis virus (IBV). The purified and nonpurified virus used as antigen, the capture and detector a ntibodies, and the chicken hyperimmune sera were prepared and standardized for this purpose. A total of 156 sera from vaccinated and 100 from specific pathogen-free chickens with no recorded contact with the virus were tested . The respective serum titers obtained in the serum neutralization test (SN T) were compared with those obtained in the LPB-ELISA. There was a high cor relation (r(2)= 0.8926) between the two tests. The LPB-ELISA represents a s ingle test suitable for the rapid detection of antibodies against bronchiti s virus in chicken sera, with good sensitivity (88%), specificity (100%) an d agreement (95.31%).