Determination of bisphenol A in canned drinks by LC MS

A simple and rapid method using liquid chromatography/electrospray ionizati on-mass spectrometry (LC/ESI-MS) has been developed for the determination d f bisphenol A in canned drinks. LC separation was carried out on a Zorbax X DB-C18 column (150 x 2.1 mm) using 0.01% acetic acid-acetonitrile (60 : 40) as the mobile phase at a flow rate of 0.2 ml/min. The negative ionization produced a typical (M - H)(-) molecular ion of bisphenol A (m/z 227). The c alibration graph for bisphenol A was rectilinear from 10 to 1000 pg with a detection limit by selected ion monitoring (SIM) of about 5 pg. Samples wer e cleaned up on a Isolute Multimode cartridge (500 mg). The recovery of bis phenol A from beverages fortified at levels 5 and 50 ng/ml was 85.4 similar to 96.3%, with high precision. Bisphenol A concentrations of canned drinks were N.D. similar to 212.1 ng/ml. The limit of quantification of bisphenol A in canned drinks was 0.5 ng/ml, except for coffee drinks.