Effects of lipo prostaglandin E-1 on distribution of cis-diamminedichloroplatinum in lung metastasis derived from Dunn osteosarcoma cell-inoculated mouse foot-pad

This study was designed to determine the effect of lipid microspheres conta ining prostaglandin E-1 (lipo PGE(1)) on cis-diamminedichloroplatinum (CDDP ) accumulation in primary and lung metastatic lesions. Sixty mice were divi ded into four groups, depending on whether or not an intra-foot-pad injecti on of Dunn osteosarcoma cells had been administered and on whether or not a n intraperitoneal injection of lipo PGE(1) had been administered. CDDP was injected intraperitoneally into all the mice 6 weeks after the inoculation. Tumor colonies of spontaneous metastases in the left lung were found in 21 out of 30 tumor-inoculated mice at autopsy. Tissue platinum concentrations in the lungs with metastatic colonies and in the foot-pad tumors were sign ificantly higher in the lipo PGE(1)-administered mice than in those without treatment. Terminal deoxytransferase-mediated dUTP nick end labeling (TUNE L) assay showed marked localization of dying cells in the lung metastatic l esions of the lipo PGE(1)-administered mice. The results of this study show ed that pretreatment with lipo PGE(1) may augment the antitumor effects of CDDP at the tumor site. (C) 1999 Elsevier Science Ireland Ltd. All rights r eserved.