Isolation and identification of Mycoplasma genitalium from high risk populations of sexually transmitted diseases in China

Objective To investigate (Mg) infection in high risk transmitted diseases ( STDs) culture, Mg-DNA detection, electron microscope (EM) microscope (AFM). Mycoplasma genitalium populations of sexually in China by means of observa tion under an and an atomic force Methods 227 urogenital specimens taken from both patients in clinics and hi gh risk populations of STDs in Beijing, Nanjing and Shanghai areas were cul tured in SP-4 medium for isolation of Mg. Suspicious positive strains were identified first by PCR technique which could amplify the special DNA fragm ents of Mg adhesion protein (Mg-Pa) and 16S-rRNA sequences. The morphologic features of Mg were observed under EM and AFM. Results Eight strains were isolated from the 227 specimens, the total posit ive rate was 3.52%. The isolated strains met the criteria of growth and met abolic conditions required by Mg. Specific fragments of Mg-DNA (374 bp base d on Mg-Pa and 402 bp on 16S-rRNA primers, respectively) could be amplified from both the isolates and the genital secretions. Under EM and AFM, Mg sh owed different shapes, including flask, pear, spindle and globe shape. The typical shape of Mg was flask or pear shape with projecting neck portion an d broadened terminal structure under EM and with constrictive ring between the neck and body under AFM. Conclusion Mg infection exists in high risk populations of STDs in China. W e highly suggest the use of AFM in dermatological study.