NILR-1, a novel immunoglobulin-like receptor expressed by neutrophilic granulocytes, is encoded by a leukocyte receptor gene complex on rat chromosome 1
Sf. Berg et al., NILR-1, a novel immunoglobulin-like receptor expressed by neutrophilic granulocytes, is encoded by a leukocyte receptor gene complex on rat chromosome 1, EUR J IMMUN, 29(6), 1999, pp. 2000-2006
Several receptors expressed by subsets of leukocytes and with sequence homo
logy to the killer cell inhibitory receptors have recently been identified
both in man and mouse. Here we describe a rat cDNA that encodes a novel rec
eptor of this group, designated neutrophil immunoglobulin-like receptor-1 (
NILR-1). The predicted 58.7-kDa mature NILR-1 protein is a type I integral
membrane protein, with three C2-type immunoglobulin superfamily domains, a
transmembrane region devoid of charged amino acids, and a cytoplasmic tail
containing four immunoreceptor tyrosine-based inhibition motif-like regions
. NILR-1 shows greatest sequence homology to the mouse paired immunoglobuli
n-like receptor-B and members of the human leukocyte immunoglobulin-like re
ceptor/immunoglobulin-like transcript group of receptors. As shown by North
ern blot analysis, NILR-1 was transcribed by neutrophilic granulocytes. Alt
hough weaker transcription was found with a macrophage cell line, no signal
was detected with peritoneal macrophage or spleen RNA. Linkage analysis lo
calized Nilr1 to chromosome 1, closely linked to a locus encoding a rat NKp
46 orthologue. The two loci define a rat leukocyte receptor gene complex, i
n a region syntenic to human chromosome 19q13.4 and the proximal part of mo
use chromosome 7, that harbors the human and mouse leukocyte receptor gene
complexes.