Recombinant human erythropoietin stimulates vascular endothelial growth factor release by glomerular endothelial cells

Citation
K. Nitta et al., Recombinant human erythropoietin stimulates vascular endothelial growth factor release by glomerular endothelial cells, EUR J PHARM, 373(1), 1999, pp. 121-124
Citations number
16
Categorie Soggetti
Pharmacology & Toxicology
Journal title
EUROPEAN JOURNAL OF PHARMACOLOGY
ISSN journal
00142999 → ACNP
Volume
373
Issue
1
Year of publication
1999
Pages
121 - 124
Database
ISI
SICI code
0014-2999(19990528)373:1<121:RHESVE>2.0.ZU;2-D
Abstract
We designed the present study to address the question of whether recombinan t human erythropoietin stimulates DNA synthesis and vascular endothelial gr owth factor (VEGF) secretion in vitro using cultured bovine glomerular endo thelial cells (GENs). Recombinant human erythropoietin dose-dependently sti mulated the proliferation of GENs in culture, and this proliferative effect was inhibited by 1 mu g/ml anti-VEGF antiserum. The increase in VEGF conce ntrations in the supernatants containing 10 U/ml rHuEpo was abolished by in cubation with 10 mu g/ml of anti-human rHuEPO antiserum, 0.2 mu g/ml actino mycin D or 10 mu g/ml cycloheximide. Taken together, rHuEpo stimulates GEN proliferation in vitro and VEGF release from these tells is associated with stimulation of RNA-dependent DNA and protein synthesis. (C) 1999 Elsevier Science B.V. All rights reserved.