Administration of Flk2/Flt3 ligand induces expansion of human high-proliferative potential colony-forming cells in the SCID-hu mouse

Citation
R. Namikawa et al., Administration of Flk2/Flt3 ligand induces expansion of human high-proliferative potential colony-forming cells in the SCID-hu mouse, EXP HEMATOL, 27(6), 1999, pp. 1029-1037
Citations number
50
Categorie Soggetti
Cardiovascular & Hematology Research
Journal title
EXPERIMENTAL HEMATOLOGY
ISSN journal
0301472X → ACNP
Volume
27
Issue
6
Year of publication
1999
Pages
1029 - 1037
Database
ISI
SICI code
0301-472X(199906)27:6<1029:AOFLIE>2.0.ZU;2-7
Abstract
The effects of Flk2/Flt3 ligand (FL) administration on human hematopoiesis were investigated using SCID-hu mice transplanted with human fetal bone fra gments. Treatment with recombinant human FL induced significant increases i n the frequencies of the high-proliferative potential colony-forming cells and low-proliferative potential colony-forming cells in steady-state human bone marrow. FL also promoted the expansion of high-proliferative potential colony-forming cells and low-proliferative potential colony-forming cells in the human bone marrow during the recovery phase after irradiation, which was evident in increases in the frequencies as well as in the absolute num bers of colony-forming cells, Furthermore, higher percentages of CD33(+)CD1 5(-) cells were found in the marrows treated with FL as compared to that of controls, indicating that FL hastened the recovery of at least some aspect of myelopoiesis after irradiation. These results indicate that FL induces the expansion of primitive hematopoietic progenitor cells in vivo and, ther efore, may be useful in treating patients to promote an early hematopoietic recovery after cytoablative therapies. (C) 1999 International Society for Experimental Hematology, Published by Elsevier Science Inc.