Previously, we cloned telomere-associated sequences using Vectorette-PCR an
d mapped the most telomeric regions of barley chromosome arms 1S (7HS), 2S
(2HS), and 4S (4HS) (Kilian and Kleinhofs 1992). Here we report mapping 33
different telomere-associated markers (21 RFLPs and 12 PCR-based) identifyi
ng the most telomeric region of 10 out of the 14 barley chromosome arms. Ot
her telomere-associated markers mapped very close, but not at the telomere,
or were interstitial. Four markers mapped at centromeric regions. Two mark
ers, generated by PCR using a single primer based on the sequence of the ba
rley telomere repeats, mapped to the end of barley chromosome 7L (5HL) and
internally on chromosome 3L (3HL). Cloning and sequencing of PCR products f
rom the 3L (3HL) interstitial location revealed homology to the HvRT family
. Several low copy number sequences physically linked to HvRTs were also cl
oned and used as RFLP probes. These probes often mapped close to the ends o
f linkage groups. A few RFLPs mapped at centromeric regions in agreement wi
th the result of in situ hybridization of HvRT clones showing positive sign
al at the ends and around centromeres of barley chromosomes.