A sequence-ready map of the human chromosome 17p telomere

A half-YAC clone derived from human chromosome 17p was mapped at high resol ution using cosmid subclone fingerprint analysis. Colinearity of the half-Y AC with the telomeric human genomic DNA fragment was ascertained by RecA-as sisted restriction endonuclease cleavage mapping. Previously isolated and r adiation hybrid-mapped markers TEL17P37, TEL17P49, and TEL17P80 mapped 30-6 0 kb from the 17p terminus. This sequence-ready map permits high-resolution integration of genetic maps with the DNA sequences directly adjacent to th e tip of human chromosome 17p, and will provide the cloned DNA required for ascertaining the nucleotide sequence of this subtelomeric region. (C) 1999 Academic Press.