The main acidic glycosphingolipids (GSLs) of cock testis were identified as
GalCer I-3-sulfate and gangliosides GM4, GM3, GD3 and GT3. They contained
N-acetylneuraminic acid as the major sialic acid, and ceramides composed ma
inly of sphingosine (dl8:1) and C18-24 non-hydroxy fatty acids. Appreciable
amounts of hydroxy fatty acids were detected only in the GM4 preparation.