Trisomy 3 in gastric lymphomas of extranodal marginal zone B-cell (mucosa-associated lymphoid tissue) origin demonstrated by FISH in intact paraffin tissue sections
R. Blanco et al., Trisomy 3 in gastric lymphomas of extranodal marginal zone B-cell (mucosa-associated lymphoid tissue) origin demonstrated by FISH in intact paraffin tissue sections, HUMAN PATH, 30(6), 1999, pp. 706-711
Citations number
20
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research Diagnosis & Treatment
Some reports have indicated that trisomy 3 represents a characteristic chro
mosomal abnormality found in lymphomas arising in mucosa-associated lymphoi
d tissues (MALT)/extranodal marginal zone B-cells (MZBC). Traditional cytog
enetic analysis of metaphase preparations is cumbersome and not always poss
ible, especially in those situations in which the diagnosis in not suspecte
d before a biopsy. Our aim is to use a relatively simple method to evaluate
trisomy 3 in paraffin-embedded, formalin-fixed tissue, using fluorescence
in situ hybridization (FISH) on intact tissue sections. Formalin-fixed, par
affin-embedded archival tissues from 30 cases (27 lymphoma and 3 chronic ga
stritis cases) were hybridized with a chromosome 3 specific alpha-satellite
probe (ONCOR, Gaithersburg, MD). Three of four cases of gastric MZBC/MALT
lymphoma revealed trisomy 3. Ten cases of lymphoma of possible or probable
MZBC origin were examined, and four revealed trisomy 3. Five of 13 non-MZBC
lymphomas revealed trisomy 3. None of the chronic gastritis cases nor norm
al tonsil cases revealed trisomy 3. Our results, using a different methodol
ogical approach, confirm the findings of others that trisomy 3 is an abnorm
ality found in a significant proportion of lymphomas of MZBC origin. Our ap
proach also makes possible interphase cytogenetic analysis (by FISH) of rou
tinely processed formalin-fixed, paraffin-embedded tissues, without the nee
d to disaggregate cells. It thus may facilitate genetic analysis on specime
ns previously deemed unsuitable for such analysis, particularly when tissue
quantity is limited. Copyright (C) 1999 by W.B. Saunders Company.