Fluorescence microlymphography of the upper extremities. Evaluation with anew computer programme

Background. To evaluate the initial lymphatics of the superficial skin in h ealthy volunteers using fluorescence microlymphography and to establish con trols values for comparison with lymphedema patients. Methods. Fluorescence microlymphography was performed on the hand dorsum, o n the lower and the upper arm in 12 healthy subjects (58.7+/-8.0 years). At each of these sites 10 mu l FITC-dextran was injected subepidermally using a steel cannula. The studies were recorded on video tape using a fluoresce nce microscope and a CCD video camera. Final magnification was 24 and 62. T he maximum spread of the fluorescent contrast medium was measured 10 minute s after injection. The area of the visualized lymph capillaries was determi ned using a computer programme. Setting. University Hospital, Department of Medicine, Division of Vascular Medicine (Angiology). Results. The mean area of the visualised lymph capillary network 95.3+/-41. 3 mm(2) (42-174 mm) at the upper and 89.4+/-45.5 mm(2) (44-171 mm). The max imum spread was 4.8+/-3.5 mm (1.9-13.6 mm) and 4.4+/-3.7 mm, respectively. The mean diameter of the lymph capillaries was 84.1+/-19.9 mu m and 75.5+/- 14.8 mu m, respectively. Conclusions. The extension of the lymph capillary network at the upper and lower arm are comparable to those at the lower extremities. Considering the two-dimensional nature and the irregular shape of the network the area mea surement seems to be more appropriate than the maximum spread in one direct ion.