Preliminary investigation of resistance of plasmid DNA to neon ion beams using transformation into recipient Escherichia coli cells.

Authors
Imamura, M Seki, T Tamai, T Nakagawa, T Nishimura, A Yamashiki, N Inoue, K Imamura, M Akagi, K Sawada, S Kasahara-Imamura, M Okamura, A Takimoto, K Watanabe, H Ohnishi, T Harada, K
Citation
M. Imamura et al., Preliminary investigation of resistance of plasmid DNA to neon ion beams using transformation into recipient Escherichia coli cells., INT J MOL M, 4(1), 1999, pp. 57-59
Citations number
14
Categorie Soggetti
Medical Research General Topics
Journal title
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
ISSN journal
11073756 → ACNP
Volume
4
Issue
1
Year of publication
1999
Pages
57 - 59
Database
ISI
SICI code
1107-3756(199907)4:1<57:PIOROP>2.0.ZU;2-1
Abstract
The sensitivity of the vector plasmid pKmK8 DNA to neon ion beams was studi ed under dry conditions. This plasmid contains the cloning vector pJKKmf(-) and a 3.6 kbp segment encoding the Escherichia coli clp gene that can be u sed in mutagenesis analysis. The survival curve of the plasmid indicated an exponential profile and a D-10 Value of about 16 kGy in the E. coli wild-t ype strain for DNA repair capability. This was similar to the D-10 value fo r the shuttle vector plasmid pZ189 DNA. Therefore, it was assumed that the excision repair system in E. coli was not effective for repairing DNA lesio ns induced by irradiation with heavy ion beams.