M. Maeda-yamamoto et al., Effects of tea polyphenols on the invasion and matrix metalloproteinases activities of human fibrosarcoma HT1080 cells, J AGR FOOD, 47(6), 1999, pp. 2350-2354
The effects of tea polyphenols on the invasion of highly metastatic human f
ibrosarcoma HT1080 cells through a monolayer of human umbilical vein endoth
elial cells (HUVECs) and the accompanying basal membrane were investigated.
Among the tea polyphenols tested, epicatechin gallate (ECg), epigallocatec
hin gallate (EGCg), and theaflavin strongly suppressed the invasion of HT10
80 cells into the monolayer of HUVECs/gelatin membrane, whereas epicatechin
, epigallocatechin, tea flavonols, tea flavones, and gallate derivatives ha
d no effect. Both theaflavin-digallate and theasinensin D showed a weak inv
asion inhibitory effect. ECg significantly inhibited the invasion without c
ytotoxicity against cancer cells and HUVECs. Ester-type catechins (ECg and
EGCg) and theaflavin strongly suppressed the gelatin degradation mediated b
y matrix metalloproteinase (MMP) 2 and MMP-9, which were secreted into the
conditioned medium of HT1080 cells. In conclusion, among the tea polyphenol
s tested, ECg was considered to be the agent with the most potential antime
tastasis activity because it inhibited invasion in the absence of cytotoxic
ity.