Am. Indrawati,"van Loey et al., Soybean lipoxygenase inactivation by pressure at subzero and elevated temperatures, J AGR FOOD, 47(6), 1999, pp. 2468-2474
Soybean lipoxygenase (LOX) inactivation [0.4 mg/mL in Tris-HCl buffer (0.01
M, pH 9)] was studied quantitatively under constant pressure (up to 650 MP
a) and temperature (-15 to 68 degrees C) conditions and kinetically charact
erized by rate constants, activation energies, and activation volumes. The
irreversible LOX inactivation followed a first-order reaction at all pressu
re-temperature combinations tested. In the entire pressure-temperature area
studied, LOX inactivation rate constants increased with increasing pressur
e at constant temperature. On the contrary, at constant pressure, the inact
ivation rate constants showed a minimum around 30 degrees C and could be in
creased by either a temperature increase or decrease. On the basis of the c
alculated rate constants at 102 pressure-temperature combinations, an iso-r
ate contour diagram was constructed as a function of pressure and temperatu
re. The pressure-temperature dependence of the LOX inactivation rate consta
nts was described successfully using a modified kinetic model of Hawley (Bi
ochemistry 1971, 10, 2436-2442).