Use of paper as a support for immobilized peroxidase in a test method for the determination of mercury(II) traces

Authors
Muginova, SV Akovbyan, NA Shekhovtsova, TN
Citation
Sv. Muginova et al., Use of paper as a support for immobilized peroxidase in a test method for the determination of mercury(II) traces, J ANALYT CH, 54(6), 1999, pp. 569-573
Citations number
19
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF ANALYTICAL CHEMISTRY
ISSN journal
10619348 → ACNP
Volume
54
Issue
6
Year of publication
1999
Pages
569 - 573
Database
ISI
SICI code
1061-9348(199906)54:6<569:UOPAAS>2.0.ZU;2-M
Abstract
A comparative study has been performed on the activity and stability of hor seradish peroxidase preparations immobilized on different papers and their suitability for the determination of mercury(II). A test method has been de veloped for the determination of mercury(II) by its inhibiting effect on th e immobilized enzyme in the presence of thiourea in the reaction of 3,3',5, 5'-tetramethylbenzidine oxidation by hydrogen peroxide with the visual dete ction of the rate of the enzymatic process. The detection limit for mercury (II) is 5-10 pg/mL (RSD less than or equal to 4%, P = 0.95, n = 6).