Determination of leukotriene E-4 in human urine using liquid chromatography-tandem mass spectrometry

A Liquid chromatographic-tandem mass spectrometric (LC-MS-MS) method was de veloped for the quantitation of urinary leukotriene E-4 (LTE4). LTE4 and it s internal standard were extracted by solid-phase extraction and analysed u sing LC-MS-MS in the selected reaction monitoring (SRM) mode. A good linear response over the range of 10 pg. to 10 ng was demonstrated. The accuracy of added LTE4 ranged from 97.0% to 108.0% with a mean and SD of 100.6+/-2.4 %. We detected LTE4 (63.1+/-18.7 pg/mg creatinine, n=10) in healthy human u rine. This method can be used to determine LTE4 in biological samples. (C) 1999 Elsevier Science B.V. All rights reserved.