High-performance liquid chromatographic purification and capillary electrophoresis quantification of the chemokine stromal cell-derived factor-1

Chemokines are members of the chemotactic cytokines family implicated in va rious immunoregulatory functions. The CXC-chemokine stromal cell derived fa ctor-1 (SDF-1 alpha) was purified from the culture medium of murine bone ma rrow stromal cell line (MS-5) by affinity and reversed-phase liquid chromat ography. Yield and purity were assessed by capillary electrophoresis (CE) w ith reference to the human SDF-1 alpha from recombinant DNA technology. CE technique was useful to evaluate the purity of human SDF-1 alpha from chemi cal synthesis and to resolve murine and human SDF-1 alpha, differing by onl y one amino acid. Chemotactic activity of the murine SDF-1 alpha was tested on the basis of CE quantification. (C) 1999 Elsevier Science B.V. All righ ts reserved.