Identification of phosphate-regulated genes by differential expression in the UV-irradiated host system

Authors
Chuang, SE Blattner, FR
Citation
Se. Chuang et Fr. Blattner, Identification of phosphate-regulated genes by differential expression in the UV-irradiated host system, J MICROB M, 37(1), 1999, pp. 93-96
Citations number
15
Categorie Soggetti
Biology,Microbiology
Journal title
JOURNAL OF MICROBIOLOGICAL METHODS
ISSN journal
01677012 → ACNP
Volume
37
Issue
1
Year of publication
1999
Pages
93 - 96
Database
ISI
SICI code
0167-7012(199907)37:1<93:IOPGBD>2.0.ZU;2-V
Abstract
The UV-irradiated host system has been used for identifying protein product s of genes cloned in a phage vector. By starving the host cells for phospha te immediately before W-irradiation, we demonstrate that phosphate-regulate d genes can be easily identified. By employing this new technique, we also provide evidence showing that the gpsA gene might be a new member of the ph osphate starvation-inducible (psi) genes of E. coli. (C) 1999 Elsevier Scie nce B.V. All rights reserved.