Steroidogenic factor 1-DNA binding: a kinetic analysis using surface plasmon resonance

Basal expression of the glycoprotein hormone alpha-subunit gene in pituitar y gonadotrophs is partially dependent on a gonadotroph specific element (GS E) which binds the nuclear receptor, steroidogenic factor-1 (SF-1). We have used surface plasmon resonance (SPR) to determine the association (k(ass)) , dissociation (k(diss)) and affinity (K-A) constants of SF-1 binding to im mobilized oligonucleotides containing either the GSE consensus motif or a G SE mutant with a 2 bp substitution in the GSE site (GSE(MUT)). In vitro translated SF-1 protein bound the consensus GSE with a threefold i ncrease in affinity constant (P<0.01) compared with the GSE(MUT). This was due primarily to a significant increase (P<0.05) in the k(ass) for SF-1 to the GSE and a slower k(diss) (P<0 05). The binding interaction was specific and could be significantly inhibited (P<0.001) by eitheranti-SF-1 antibody or excess non-biotinylated GSE. The addition of 14 bp wild-type flanking s equences significantly reduced the affinity of SF-1 to both the GSE (P<0.05 ) and the GSE(MUT) (P<0.01). This was due to a significant (P<0.01) decreas e in k(ass) for the wild-type and mutant long oligonucleotides compared wit h the short GSE. Nuclear extracts from alpha T3-1 gonadotroph cells also bo und the GSE and GSE(MUT), giving k(diss) values which were two- to threefol d slower than those obtained with in vitro translated SF-1. Thus, SPR is a powerful technique for examining kinetic interaction between SF-1 and its binding site, and is able to demonstrate the effects of mutat ions and flanking sequences on that interaction.