E. Noroozian et al., Determination of roquefortine C in blue cheese using on-line column-switching liquid chromatography (vol 17, pg 1215, 1998), J PHARM B, 20(3), 1999, pp. 609
A method is described for the determination of roquefortine C in (blue) che
ese. After liquid-liquid extraction with a mixture of hydrochloric acid and
methanol, and filtration, an aliquot is analysed using column-switching re
versed-phase liquid chromatography. The recovery of roquefortine C in Fetta
cheese is about 85%, the calibration curve is linear from 10 to 2500 ng g(
-1) (r(2) = 0.998), and the detection limit is about 10 ng g(-1). In differ
ent batches of Danish Blue concentrations of 1000-2000 ng g(-1) of roquefor
tine C are found. As regards the stability of roquefortine C its half-life
in diffuse daylight is ca. 50 min, while after irradiation with ultraviolet
light, it is about 10 min. (C) 1999 Elsevier Science B.V. All rights reser
ved.