Chemoenzymatic preparation of the novel antifolate thymidylate synthase inhibitor N-(4-{N-[(6S)-2-methyl-4-oxo-3,4,7,8-tetrahydro-6H-cyclopenta [g]quinazolin-6-yl]-N-(prop-2-ynyl)amino}-benzoyl)-L-glutamic acid and its glutamyl cleavage product

5-Aminoindane was converted in six steps to the cydopenta[g]quinazoline ket one 13. Condensation of 13 with diethyl 4-aminobenzoyl-L-glutamate, followe d by in situ reduction, produced the secondary amine 15. N-Propargylation o f 15, followed by deprotection, gave the diacid 17 as a mixture of diastere oisomers. Treatment of 17 with the bacterial enzyme carboxypeptidase G(2) r esulted in removal of the L-glutamic acid residue from (6R)-17 to give a ch romatographically separable mixture of the monoacid 18 and the antifolate 5 [(6S)-17], which was assayed as an inhibitor of thymidylate synthase (K-i( app) = 3 nM). Treatment of isolated diacid 5 with carboxypeptidase G(2) pro duced the monoacid 19 in ca. 98% enantiomeric excess. The (6S) stereochemis try of compound 19 has been established by X-ray crystal structure determin ation of the amide derivative 24.