S. Balakrishnan et Sb. Rao, Cytogenetic analysis of peripheral blood lymphocytes of occupational workers exposed to low levels of ionising radiation, MUT RES-GTE, 442(1), 1999, pp. 37-42
Citations number
36
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
The incidence of chromosomal aberrations was analysed in peripheral blood l
ymphocytes of occupationally exposed people having cumulative doses of 500
mSv. The exposed individuals showed higher frequencies of dicentrics as wel
l as acentrics than normal controls. Absorbed radiation dose was calculated
by using in vitro dose response curve established for Cobalt-60 gamma rays
. In the control constituting 17 healthy individuals, two dicentrics were d
etected among 3700 metaphases analysed. In the exposed group 27 dicentrics
and one centric ring was detected among 8400 metaphases analysed. Due to sm
all number of dicentrics scored in each individual, the dose estimate suffe
rs from a large statistical uncertainty. The collective dose was found to b
e 1.89 Gy. This is in good agreement with the corrected physical doses, ass
uming a mean life of 10 years for the disappearance of lymphocytes. The phy
sical doses accumulated during the last 10 years of occupation were also in
good agreement with the biological dose estimate. (C) 1999 Elsevier Scienc
e B.V. All rights reserved.