Time until initiation of tumor growth is an effective measure of the anti-angiogenic effect of TNP-470 on human glioblastoma in nude mice

Citation
M. Kragh et al., Time until initiation of tumor growth is an effective measure of the anti-angiogenic effect of TNP-470 on human glioblastoma in nude mice, ONCOL REP, 6(4), 1999, pp. 759-762
Citations number
21
Categorie Soggetti
Oncology
Journal title
ONCOLOGY REPORTS
ISSN journal
1021335X → ACNP
Volume
6
Issue
4
Year of publication
1999
Pages
759 - 762
Database
ISI
SICI code
1021-335X(199907/08)6:4<759:TUIOTG>2.0.ZU;2-Q
Abstract
We examined the effect of the anti-angiogenic compound TNP-470 on early tum or growth characteristics following subcutaneous implantation of 1 mm(3) ti ssue blocks of human glioblastoma U87, in nude mice. The mice received dail y injections with TNP-470, 7 mg/kg, from one day before until either 3, 7, 11, or 15 days after inoculation. The time from inoculation until initiatio n of exponential tumor growth was determined along with the post-therapeuti c growth delay and the initial tumor doubling time (TD) for each individual tumor (n=103) on the basis of tumor volume growth curves. We found that: i ) the onset of growth of U87 xenografts was effectively inhibited by concur rent treatment with TNP-470 beyond the first three days after inoculation, ii) this effect was fully reversible upon termination of therapy, and iii) the posttherapeutic growth delay was independent of the accumulated dose. T hese findings demonstrate that the in vivo effect of TNP-470 on tumor growt h is tumor inhibitory rather than cytotoxic. The lack of effect of the anti -angiogenic compound, TNP-470, in the early 3-day schedule is consistent wi th the existence of an early avascular phase which precede the angiogenesis -dependent tumor growth.