Jl. Spouge et Sp. Layne, A practical method for simultaneously determining the effective burst sizes and cycle times of viruses, P NAS US, 96(12), 1999, pp. 7017-7022
Citations number
21
Categorie Soggetti
Multidisciplinary
Journal title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
We describe combined analytic and experimental methods for determining repr
oductive statistics from time-series data. Our computational methods derive
four fundamental measures from laboratory experiments: (i) average number
of viral daughters; (ii) mean viral cycle time; (iii) standard deviation of
the viral cycling time; and (iv) viral doubling time. Taken together, thes
e four reproductive statistics characterize "age-specific fertility," a qua
ntity that provides complete information on the reproduction of the average
viral particle. In this paper, we emphasize applications relating to HIV a
nd experiments for assessing cellular tropism, viral phenotypes, antiviral
drugs, humoral immunity, and cytotoxic cellular immunity, Nevertheless, our
method is quite flexible and applicable to the evaluation of drugs against
bacterial, fungal, and parasitic infections, antineoplastic agents against
cancer cells, and perturbations involving pest and wildlife releases in ec
osystems.