Fluorescence chemosensors with pyrene and their interaction with nucleotide phosphate

A group of fluorescence chemosensor with pyrene, compounds ( I), ( II) and ( III), were synthesized. The fluorescence spectra and the lifetime of thes e compounds were carefully measured. The fluorescence quenching spectra of pyrenyl butyric acid, compounds ( I), ( II) and ( III) by different nucleot ide phosphates, AMP ADP, ATF, dTTP, were also recorded and studied. The que nching and the stability constants were calculated Ly Stem-Volmer equation and eq. (2), respectively. The mechanism of interaction between fluorescenc e chemosensor and nucleotide phosphate was didscussed based on the comparis on of the results obtained with the CPK model of Free molecules of these co mpounds in the ground state.