Enrichment and separation of rebaudioside A from stevia glycosides by a novel adsorbent with pyridyl group

The adsorptive capacity and selectivity of a novel adsorbent with pyridyl g roup toward stevia glycosides were studied. The effect of polarity and phys ical structure of the sorbent on the selectivity was investigated in detail . Two separation methods were applied in the enrichment of rebaudioside A(R A). They were selective elution using methanol or ethanol solution as solve nt, and dynamic chromatographic separation using pyridyl resin with high se lectivity. Results show that the chromatographic separation method can effe ctively enrich RA from ste,ia glycoside with high content of stevioside.