Photochemical fluorescence enhancement of the terbium-lomefloxacin complexand its application

The sensitized fluorescence intensity of the terbium ion (Tb3+) can be nota bly enhanced after the Tb3+ lomefloxacin(LFLX) complex system was irradiate d by 365nm ultraviolet light. A photochemical reaction occurs to the irradi ated Tb3+ - LFLX complex. A new Tb3+ system with intense fluorescence is ob tained. On this basis a new sensitive and selective photochemical fluorimet ry for the determination of LFLX was established. Under the optimal experim ental conditions, the linear range of the determination is 2.0-500 x 10(-8) mol l(-1) for LFLX, and the detection limit is 6.0 x 10(-9) mol l(-1). Wit hout any pre-treatment the recoveries of LFLX in human urine and serum were determined. (C) 1999 Elsevier Science B.V. All rights reserved.