A fatty acid binding protein from Fasciola hepatica induced protection in C57/BL mice from challenge infection with Schistosoma bovis

Three strains of mice (NMRI, C57/BL, BALB/c) were each immunized with a 12 kDa purified, native Fasciola hepatica fatty acid binding protein (Fh12) an d challenged percutaneously with Schistosoma bovis cercariae. C57/BL mice i mmunized with Fh12 had significant reductions in S. bovis worm burden recov eries (96 and 87% reductions over controls in two separate experiments). Wh en using NMRI or BALB/c mice, Fh12 alone or in Freund's adjuvant failed to induce significant protection against S. bovis. In C57/BL mice vaccinated a gainst Fh12, antibodies to the IgG2a isotype, but not to the IgG1 isotype, increased by 2 weeks after the second immunization and remained high throug h 8 weeks of S. bovis infection. Antibodies to S. bovis increased after 4 w eeks of infection. Regarding cytokine production by spleen mononuclear cell s, C57/BL mice vaccinated with Fh12 in adjuvant, and having the highest pro tective response against challenge infection with S. bovis, had an increase of IFN gamma production with Concanavalin A but no increase of IL-4 in sim ilarly stimulated cells. These results suggest that the protection obtained in this group of mice is mediated by a Th1 immune response. (C) 1999 Elsev ier Science B.V. All rights reserved.