Crystallization and preliminary X-ray studies of the basic lectin from theseeds of Artocarpus hirsuta

The basic lectin from Artocarpus hirsuta specific towards methyl ol-galacto se has been purified and crystallized using the hanging-drop vapour-diffusi on method with ammonium sulfate as precipitant. Three different crystal for ms, orthorhombic I, orthorhombic II and hexagonal, were grown under the sam e crystallization conditions. The orthorhombic forms belonged to space grou p P2(1)2(1)2(1) with unit-cell dimensions a = 92.9, b = 99.8, c = 166.2 Ang strom and a = 89.9, b = 121.9, c = 131.6 Angstrom, respectively. The unit-c ell dimensions of the hexagonal form were a = b = 84.1 and c = 271.7 Angstr om and the space group was P6(1)22.