Optimized analysis of intracellular adenosine and guanosine phosphates in Escherichia coli

Authors
Meyer, S Noisommit-Rizzi, N Reuss, M Neubauer, P
Citation
S. Meyer et al., Optimized analysis of intracellular adenosine and guanosine phosphates in Escherichia coli, ANALYT BIOC, 271(1), 1999, pp. 43-52
Citations number
19
Categorie Soggetti
Biochemistry & Biophysics
Journal title
ANALYTICAL BIOCHEMISTRY
ISSN journal
00032697 → ACNP
Volume
271
Issue
1
Year of publication
1999
Pages
43 - 52
Database
ISI
SICI code
0003-2697(19990615)271:1<43:OAOIAA>2.0.ZU;2-Z
Abstract
To investigate the intracellular concentrations of adenosine phosphates in Escherichia coli, especially during bioreactor cultivations, a method that enables reproducible determination of adenosine phosphates in culture solut ions containing at least 0.25 g dry cell weight/L has been developed. The d etection limits of AMP, ADP, and ATP were found to be as low as 1 pmol. The method involves fast sampling, instantaneous inactivation of cell metaboli sm, extraction of nucleotides, and quantitative analysis by ion-pair revers ed-phase HPLC. (C) 1999 Academic Press.