Enzyme linked immunoassay (ELISA) for lecithin : cholesterol acyltransferase (LCAT)

Several immunoassay models, including sandwich ELISA, solid phase ELISA and sink immunoassay and antibody combinations were investigated to develop an ELISA assay for LCAT with an appropriate linear range and sensitivity. Sol id phase immunoassays were found to be most suitable for measuring LCAT fro m cell culture medium and in partially purified preparations. The immunoass ays were analyzed for matrix interference, recovery studies, intrarun preci sion and inter-run precision. These studies have identified a reliable meth od for measuring LCAT in purified preparations and cell culture media, and provide the foundation for further development of immunoassays for clinical application.