Several immunoassay models, including sandwich ELISA, solid phase ELISA and
sink immunoassay and antibody combinations were investigated to develop an
ELISA assay for LCAT with an appropriate linear range and sensitivity. Sol
id phase immunoassays were found to be most suitable for measuring LCAT fro
m cell culture medium and in partially purified preparations. The immunoass
ays were analyzed for matrix interference, recovery studies, intrarun preci
sion and inter-run precision. These studies have identified a reliable meth
od for measuring LCAT in purified preparations and cell culture media, and
provide the foundation for further development of immunoassays for clinical
application.