Ma. Cissell et R. Chalkley, Characterization of a kidney-specific pattern of chromatin structure in the rat phosphoenolpyruvate carboxykinase gene, BBA-GENE ST, 1445(3), 1999, pp. 299-313
Citations number
58
Categorie Soggetti
Molecular Biology & Genetics
Journal title
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
The kidney-specific chromatin structure of the phosphoenolpyruvate carboxyk
inase (PEPCK) gene was examined and compared to that of the liver. Kidney n
uclear extracts were found to lack a liver-enriched factor, pepA, that bind
s to HSS A, a distal enhancer of the PEPCK gene that may be involved in ope
ning the chromatin domain of the PEPCK gene in the liver. To begin the char
acterization of the kidney-specific chromatin structure of the PEPCK gene,
nuclease hypersensitive sites (HSS) were mapped by indirect end-labeling an
alysis in proximal tubules from control rats, proximal tubules from acidoti
c rats which express induced levels of PEPCK, and NRK52E cells, a rat kidne
y epithelial cell line which does not express the PEPCK gene. A subset of H
SS, at -400/+1 over the proximal promoter and at +1900 within the coding re
gion, correlate with kidney-specific PEPCK expression Two other HSS, at -3.
1 kb and +6.2 kb, are detected in kidney cells regardless of PEPCK expressi
on. The HSS at -4800, -1240, and +4650, previously identified in PEPCK-expr
essing liver cells, were not observed in the kidney. As in the liver, the p
attern of hypersensitivity in the kidney does not change by altering the ra
te of transcription. (C) 1999 Elsevier Science B.V. All rights reserved.