Tissue-specific patterns of changes in 3,5,3 '-triiodo-L-thyronine concentrations in thyroidectomized rats infused with increasing doses of the hormone. Which are the regulatory mechanisms?
Hf. Escobar-morreale et al., Tissue-specific patterns of changes in 3,5,3 '-triiodo-L-thyronine concentrations in thyroidectomized rats infused with increasing doses of the hormone. Which are the regulatory mechanisms?, BIOCHIMIE, 81(5), 1999, pp. 453-462
We have measured 3,5,3'triiodothyronine (T-3) in 12 tissues from thyroidect
omized (Tx) rats infused with increasing doses of T-3, and related them to
their corresponding plasma levels. Young adult Wistar rats were surgically
Tx. After 4 weeks, the animals were infused with placebo or T-3 (0.25, 0.50
, 0.75, 1.00 or 2.00 mu g/100 g body weight/day). Placebo-infused intact ra
ts served as euthyroid controls. Plasma and samples of cerebral cortex, cer
ebellum, brown adipose tissue (BAT), pituitary, liver, heart, lung, kidney,
spleen, skeletal muscle, ovary and adrenal were obtained after 12-13 days
of infusion. We determined plasma T-3 and thyrotropin (TSH), and tissue T-3
and thyroxine (T-4), the latter being virtually undetectable. Results were
compared with the relationships between tissue and plasma T-3 in Tx rats o
n T-4 infusions. Most tissues presented changes which paralleled those in p
lasma T-3, irrespective of its source (infusion of T-3, or generation from
infused T-4). However, at similar plasma T-3 concentrations, cerebral corte
x, cerebellum and BAT (containing type II 5' iodothyronine deiodinase (DII)
activity), reached much lower T-3 levels in the T-3-infused Tx rats, than
in Tx rats on T-4, and required elevated plasma T-3 levels for normal tissu
e T-3. In these tissues, and in the pituitary, T-3 concentrations were alwa
ys lower than expected from plasma T-3 levels. On the contrary, the lung an
d ovary of the T-3-infused Tx rats contained more T-3 than expected from pl
asma T-3. Unexpectedly, both the ovary and adrenal attained higher tissue T
-3 concentrations in Tx rats on T-3 than on T-4 at comparable plasma T-3 le
vels. In conclusion, the patterns of changes of the concentrations of T-3 a
s a function of increasing plasma T-3 are not only tissue-specific when T-4
is provided, but also when circulating T-3 is the only source of this iodo
thyronine. Further studies are needed to identify the mechanisms involved i
n the regulation of tissue T-3 concentrations. (C) Societe francaise de bio
chimie et biologie moleculaire / Elsevier, Paris.