Integrated optical surface plasmon resonance immunoprobe for simazine detection

This paper presents the detailed design and characterisation of a regenerab le integrated optical surface plasmon resonance immunoprobe as a detector f or the triazine herbicide simazine. A sensor design theoretically optimised for use in the aqueous environment is presented and its fabrication descri bed. Experimental results on the sensitivity to changes in bulk refractive index of the analyte and on non-specific binding of ovalbumin are presented . Binding inhibition immunoassays were conducted for simazine and the lower limit of detection determined to be 0.16 mu g/l using anti-simazine IgG an tibodies and 0.11 mu g/l using anti-simazine Fab fragments. A sample test c ycle of 20 min was established. (C) 1999 Elsevier Science S.A. Al rights re served.