The demonstration of immunoreactive dystrophin and its developmental expression in perivascular astrocytes

Citation
V. Jancsik et F. Hajos, The demonstration of immunoreactive dystrophin and its developmental expression in perivascular astrocytes, BRAIN RES, 831(1-2), 1999, pp. 200-205
Citations number
23
Categorie Soggetti
Neurosciences & Behavoir
Journal title
BRAIN RESEARCH
ISSN journal
00068993 → ACNP
Volume
831
Issue
1-2
Year of publication
1999
Pages
200 - 205
Database
ISI
SICI code
0006-8993(19990612)831:1-2<200:TDOIDA>2.0.ZU;2-#
Abstract
Immunoreactivity of dystrophin family proteins was observed in the astrocyt es of the adult and immature rat hjppocampus and cerebral cortex by using D ys2, a monoclonal antibody recognizing both 427 kDa and short dystrophin is oforms. As revealed by light and electron microscopy, immunostaining of the ribosomal apparatus and of pericapillary endfeet was particularly pronounc ed in the adult. In the pericapillary astrocyte processes immunostaining ap peared between postnatal days 10 and 20, and reached the intensity seen in the adult by postnatal day 30. In the pericapillary astrocyte process, the membrane facing the endothelial basal lamina was the earliest structure to show the immunoreaction. At later stages, the pericapillary astrocyte proce ss was gradually filled up with immunoprecipitate. Findings suggest that dy strophins are expressed coinciding with the development of the blood-brain barrier, and it is assumed that they contribute to the formation of this sy stem. (C) 1999 Elsevier Science B.V. All rights reserved.