Citation
Ra. Mafra et al., PhTx4, a new class of toxins from Phoneutria nigriventer spider venom, inhibits the glutamate uptake in rat brain synaptosomes, BRAIN RES, 831(1-2), 1999, pp. 297-300
Citations number
33
Categorie Soggetti
Neurosciences & Behavoir
Journal title
BRAIN RESEARCH
ISSN journal
00068993
→ ACNP
Volume
831
Issue
1-2
Year of publication
1999
Pages
297 - 300
Database
ISI
SICI code
0006-8993(19990612)831:1-2<297:PANCOT>2.0.ZU;2-3
Abstract
We report the characterization of a new class of glutamate uptake inhibitor
s isolated from Phoneutria nigriventer venom. Glutamate transport activity
was assayed in rat cerebrocortical synaptosomes by using [H-3]-L-glutamate.
PhTx4 inhibited glutamate uptake in a dose dependent manner. The IC,, valu
e obtained was 2.35 +/- 0.9 mu g/ml which is in the observed range reported
for glutamate uptake blockers. Tx4-7, one of PhTx4 toxins, showed the stro
ngest inhibitory activity (50.3 +/- 0.69%, n = 3). (C) 1999 Elsevier Scienc
e B.V. All rights reserved.