Ultraviolet irradiation induces cyclooxygenase-2 expression in keratinocytes

Citation
K. Isoherranen et al., Ultraviolet irradiation induces cyclooxygenase-2 expression in keratinocytes, BR J DERM, 140(6), 1999, pp. 1017-1022
Citations number
35
Categorie Soggetti
Dermatology,"da verificare
Journal title
BRITISH JOURNAL OF DERMATOLOGY
ISSN journal
00070963 → ACNP
Volume
140
Issue
6
Year of publication
1999
Pages
1017 - 1022
Database
ISI
SICI code
0007-0963(199906)140:6<1017:UIICEI>2.0.ZU;2-H
Abstract
We examined the effect of ultraviolet (UV) irradiation on the expression of cyclooxygenases in cultured HaCaT keratinocytes and in human skin in vivo. UVB irradiation (10 and 50 mJ/cm(2)) and hydrogen peroxide (200 mu mol/L) increased cyclooxygenase-2 mRNA expression in HaCaT keratinocytes. No clear expression of cyclooxygenase-1 mRNA was detected in either control or stim ulated HaCaT cells. Genistein, a tyrosine kinase inhibitor, suppressed both the basal and stimulated expression of cyclooxygenase-2 in HaCaT cells. UV B-induced cyclooxygenase-2 mRNA expression was partly inhibited by the anti oxidant N-acetylcysteine and by H-7, a non-specific inhibitor of protein ki nase C. Solar-simulated irradiation (40 mJ/cm(2)) was found to induce in vi vo both cyclooxygenase-2 mRNA and protein expression in human skin, whereas the expression of cyclooxygenase-1 mRNA remained at the basal level. Our r esults show that cyclooxygenase-2 expression is induced by UV irradiation a nd suggest that tyrosine kinases and reactive oxygen intermediates are invo lved in this induction of cyclooxygenase-2.