Interaction between fluorescence chemosensors with pyrene and calf thymus DNA

The quenching spectra of fluorescence chemosensors with pyrene by calf thym us DNA were studied in this work. The fluorescence quenching of both monome r and excimer of pyrene by ctDNA were investigated. The quenching constants of fluorescence chemosensors by ctDNA from Stern-Volmer equation were comp ared as following: for monomer, compound 2>compound 1>pyrenyl burytic acid> compound 3; for excimer, compound 2>>compound 3, The stability constants of the interaction between fluorescence chemosensors and ctDNA were calculate d according to equation (2), Of which the interaction between fluorescence chemosensors and ctDNA has been proposed according to their molecular struc ture, configuration, atom-atom distances and molecular size. The results ob tained were discussed preliminarily.