H. Yokoyama et al., In vivo simultaneous monitoring by Pt-disk microelectrodes of intracerebral hydrogen peroxide and dopamine in rats, CHEM LETT, (6), 1999, pp. 497-498
A technique was developed for simultaneous monitoring of temporal changes i
n hydrogen peroxide (H2O2) and dopamine (DA) levels by using Pt-disk microe
lectrodes ( phi 30 mu m). H2O2 and DA were determined by differential doubl
e-pulse amperometry (for H2O2; 1st step, 750 mV, 1000 ms; 2nd step, 1100 mV
, 1000 ms: far DA; 1st step, 150 mV, 600 ms; 2nd step, 250 mV, 50 ms). The
electrode for H2O2 or DA is capable of determining each target substance an
d these substances do not interfere with each other. For an in vivo applica
tion, the electrodes were implanted into the striatum of rats and used succ
essfully to monitor intrastriatal changes in H2O2 and DA simultaneously aft
er intraperitoneal injection of methamphetamine while the animals were movi
ng freely.