Calcium phosphate formation on highly-oriented collagen fibrils

Calcium phosphate formation on collagen fibrils was studied. Collagen fibri ls deposited from a collagen solution were insolubilized by UV-irradiation. The collagen fibrils looked to be precipitated and arranged in one directi on in a narrow space due to convection stream from bottom to top of test tu be. Highly-oriented collagen fibrils precipitated were soaked in simulated body fluid (SBF) solution at 36.5 degrees C. After immersion of SBF solutio n at 36.5 degrees C for 3 days, calcium phosphate was formed on insolubiliz ed collagen fibrils. In contrast, no calcium phosphate was formed on collag en fibrils without insolubilization treatment.