Ribosomal DNA location in the scarab beetle Thorectes intermedius (Costa) (Coleoptera : Geotrupidae) using banding and fluorescent in-situ hybridization

Authors
Vitturi, R Colomba, MS Barbieri, R Zunino, M
Citation
R. Vitturi et al., Ribosomal DNA location in the scarab beetle Thorectes intermedius (Costa) (Coleoptera : Geotrupidae) using banding and fluorescent in-situ hybridization, CHROMOS RES, 7(4), 1999, pp. 255-260
Citations number
29
Categorie Soggetti
Molecular Biology & Genetics
Journal title
CHROMOSOME RESEARCH
ISSN journal
09673849 → ACNP
Volume
7
Issue
4
Year of publication
1999
Pages
255 - 260
Database
ISI
SICI code
0967-3849(199906)7:4<255:RDLITS>2.0.ZU;2-4
Abstract
Mitotic metaphase chromosomes of the scarab beetle Thorectes intermedius (C osta) (Coleoptera Scarabaeoidea: Geotrupidae) were analyzed using various b anding methods and fluorescent in-situ hybridization (FISH) with a ribosoma l probe. The results obtained indicate that silver and CMA(3) staining are unable to localize the chromosome sites of nucleolar organizer regions (NOR s). Such an inadequacy is a consequence of the extensive silver and CMA(3) stainability of both constitutive heterochromatin and heterochromatin assoc iated to the NORs.