The amino acid sequence of a lamprey (Entosphenus japonicus) liver fatty acid-binding protein identified its close relationship to cardiac fatty acid-binding proteins of Mammalia
K. Baba et al., The amino acid sequence of a lamprey (Entosphenus japonicus) liver fatty acid-binding protein identified its close relationship to cardiac fatty acid-binding proteins of Mammalia, COMP BIOC B, 123(2), 1999, pp. 223-228
Citations number
25
Categorie Soggetti
Biochemistry & Biophysics
Journal title
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
Fatty acid-binding proteins (FABPs) constitute a large, tissue-specific mul
tigene family in animals. While liver FABPs of Aves, Reptilia. and Amphibia
are close homologs of mammalian liver FABPs, the shark liver protein belon
gs to the mammalian heart FABP subfamily. We purified an FABP from the live
r of lamprey, Entosphenus japonicus, by gel filtration and anion-exchange c
hromatography. Amino acid sequences determined for lysyl endopeptidase pept
ides covered nearly the entire molecule except for N- and C-terminal short
segments. Examination of the phylogenetic relationship from the sequence id
entified the lamprey liver protein as a member of mammalian heart-type FABP
s. Since livers of bony fish contain both hepatic and cardiac FABPs, expres
sion of the FABP gene in the vertebrate liver appears to change from the he
art-type to liver-type within Pisciformes. with bony fish being in a transi
tion stage. This may be related to the evolutionary and physiological diver
gence of liver function and lipid metabolism of these lower vertebrates. (C
) 1999 Elsevier Science Inc. All rights reserved.