In the present communication a personal computer control methodology for pH
data acquisition and analysis in biological systems is reported. The instr
umental control, acquisition, storage, processing and presentation of the e
xperimental data are provided by a data acquisition board, a graphical prog
ramming software and numerical analysis,'graphics software. The major objec
tive of this work is to improve the performance and flexibility of the pers
onal computer acquisition system compared with traditional approaches depen
ding on potentiometric recorders. In particular. virtual instruments for in
terfacing pH meters of different brands to a personal computer and for meas
uring proton changes in lightly buffered solutions during enzymatic reactio
ns are provided. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved
.