In vivo synthesis of complex N-glycans by expression of human N-acetylglucosaminyltransferase I in the filamentous fungus Trichoderma reesei

Authors
Maras, M De Bruyn, A Vervecken, W Uusitalo, J Penttila, M Busson, R Herdewijn, P Contreras, R
Citation
M. Maras et al., In vivo synthesis of complex N-glycans by expression of human N-acetylglucosaminyltransferase I in the filamentous fungus Trichoderma reesei, FEBS LETTER, 452(3), 1999, pp. 365-370
Citations number
28
Categorie Soggetti
Biochemistry & Biophysics
Journal title
FEBS LETTERS
ISSN journal
00145793 → ACNP
Volume
452
Issue
3
Year of publication
1999
Pages
365 - 370
Database
ISI
SICI code
0014-5793(19990611)452:3<365:IVSOCN>2.0.ZU;2-N
Abstract
The human N-acetylglucosaminyltransferase I gene was introduced in the geno me of Trichoderma reesei strain VTT-D-80133, Expression was studied after i nduction from the cellobiohydrolase I promoter. Successful in vivo transfer of GlcNAc was demonstrated by analyzing the neutral N-glycans which were s ynthesized on cellobiohydrolase I. Final proof of the formation of GlcNAcMa n(5)GlcNAc(2) was obtained by NMR analysis. (C) 1999 Federation of European Biochemical Societies.